• Catalog Number:

  E1A7045A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human c-Jun around the phosphorylation site of Serine 63.

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P05412

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000

• Specificity Sensitivity:

  c-Jun (Phospho-Ser63) Antibody detects endogenous levels of c-Jun only when phosphorylated at Serine 63.

• References:

  Wei W, et al. (2005). Cancer Cell. 8(1): 25-33. Sevilla A, et al. (2004). Oncogene.23(55): 8950-8958. Li L, et al. (2004). J Biol Chem.279(6): 4058-4065. Hurd C, et al. (2002). Oncogene.21(14): 2154-2160.

Immunohistochemical analysis of paraffin-embedded breast carcinoma, using c-Jun (Phospho-Ser63) Antibody. Left: Untreated; Right: Treated with synthesized phosphopeptide.

Western blot analysis of extracts using c-Jun (Phospho-Ser63) Antibody. Line1: The extracts from NIH/3T3 cells untreated; Line2: The extracts from NIH/3T3 cells treated with UV.