• Catalog Number:

  E011064-2

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human BCL-2 around the phosphorylation site of threonine 56 (G-H-TP-P-H).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human

• SwissProtNo:

  P10415

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100

• Specificity Sensitivity:

  BCL-2 (phospho-Thr56) antibody detects endogenous levels of BCL-2 only when phosphorylated at threonine 56.

• References:

  Ling, Y. H. et al. (1998) J. Biol. Chem. 273, 18984-18991. Huang, S.J. and Cidlowski, J.A. (2002) FASEB 16, 825-832. Deng, X. et al. (2001) J. Biol. Chem. 276, 23681-23688. Huang ST,et al. (2002) FASEB J Jun; 16(8): 825-32. Yamamoto, K. et al. (1999) Mol. Cell. Biol. 19, 8469-8478.

P-Peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using BCL-2 (phospho-Thr56) antibody (E011064).

Western blot analysis of extracts from MDA435 cells treated with sorbtiol (0.4M, 30min) using BCL-2 (phospho-Thr56) antibody (E011064).