• Catalog Number:

  E1A7217A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human SMC1 around the phosphorylation site of serine 957 (G-S-SP-Q-G).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse

• SwissProtNo:

  Q14683

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000

• Other Names:

  Chromosome segregation protein SmcB; DXS423E; KIAA0178; Sb1.8; SB1.8/DXS423E protein; SM1A; SMC1A; SMC1alpha protein; SMC1L1; Structural maintenance of chromosome 1-like 1 protein

• Specificity Sensitivity:

  SMC1 (Phospho-Ser957) antibody detects endogenous levels of SMC1 only when phosphorylated at serine 957.

• References:

  Rocques P.J., Hum. Mol. Genet. 4:243-249(1995). Nagase T., DNA Res. 3:17-24(1996). Nakajima D., DNA Res. 9:99-106(2002).

• All Sites:

  Human: Ser957; Mouse: Ser957

Western blot analysis of extracts from HUVEC cells treated with EGF (200ng/ml, 5mins) and HeLa cells treated with EGF (200ng/ml, 15mins), using SMC1 (Phospho-Ser957) antibody.