Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human Integrin β3 around the phosphorylation site of tyrosine 773 (P-L-YP-K-E).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Reactivity:
Human, Mouse
SwissProtNo:
P05106
Applications:
WB: 1:500~1:1000 IHC: 1:50~1:100
Specificity Sensitivity:
Integrin β3 (phospho-Tyr773) antibody detects endogenous levels of Integrin β3 only when phosphorylated at tyrosine 773.
References:
Chandhoke SK, et al. (2004) J Cell Sci.117 (Pt 8): 1431-1441.
Datta A, et al. (2002) J Biol Chem.277 (6): 3943-3949.
Law DA, et al. (1996) J Biol Chem.271 (18): 10811-10815.
P-Peptide - +
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Integrin β3 (phospho-Tyr773) antibody (E011060).
Western blot analysis of extracts from HL-60 cells using Integrin β3 (phospho-Tyr773) antibody.