• Catalog Number:

  E011183-2

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human AMPK1 around the phosphorylation site of threonine 174 (L-R-TP-S-C).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  Q13131

• Applications:

  IHC: 1:50-1:100

• Specificity Sensitivity:

  AMPK1(Phospho-Thr174) Antibody detects endogenous levels of AMPK1 only when phosphorylated at threonine 174.

• References:

  Hurley RL, et al. (2005) J Biol Chem. Aug 12; 280(32): 29060-29066 Woods A, et al. (2003) Curr Biol. Nov 11; 13(22): 2004-2008 Nielsen JN, et al. (2003) J Appl Physiol. Feb; 94(2): 631-641 Da Silva Xavier G, et al. (2000) Proc Natl Acad Sci U S A. Apr 11; 97(8): 4023-4028.

P-Peptide - + Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue, using AMPK1 (Phospho-Thr174) Antibody (E011183).