• Catalog Number:

  E011056-1

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human PTEN around the phosphorylation site of serine 380 and threonine 382/383 (R-Y-SP-D-TP-TP-D-S).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P60484

• Applications:

  WB: 1:500~1:1000 IHC:1:50~100 IF:1:100~1:200

• Specificity Sensitivity:

  PTEN (phospho-Ser380/Thr382/Thr383) antibody detects endogenous levels of PTEN only when phosphorylated at serine 380 and threonine 382/383.

• References:

  Al-Khouri AM, et al. (2005) J Biol Chem. 280(42):35195-35202. Torres J, et al. (2001) J Biol Chem. 276(2): 993-998. Vazquez F, et al. (2000) Mol Cell Biol. 20(14): 5010-5018.

P-Peptide - + Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue, using PTEN (phospho-Ser380/Thr382/Thr383) antibody (E011056).

Western blot analysis of extract from HT29 cells untreated or treated with calyculin A, using PTEN (phospho-Ser380/Thr382/Thr383) antibody (E011056).