• Catalog Number:

  E1A8041A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human HSF1 around the phosphorylation site of serine 121 (V-T-SP-V-S).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody againstnon-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%)

• SwissProtNo:

  Q00613

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000

• Other Names:

  Heat shock factor protein 1; Heat shock transcription factor 1; HSF 1; HSTF 1; HSTF1

• Specificity Sensitivity:

  HSF1 (Phospho-Ser121) antibody detects endogenous levels of HSF1 only when phosphorylated at serine 121.

• References:

  Rabindran S.K., Proc. Natl. Acad. Sci. U.S.A. 88:6906-6910(1991). Ota T., Nat. Genet. 36:40-45(2004). Kalnine N., Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.

• All Sites:

  Human: Ser121; Mouse: Ser121

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human brain tissue using HSF1 (Phospho-Ser121) antibody. Western blot analysis of extracts from Jurkat cells, treated with TNF (2500U/ML, 30mins), using HSF1 (Phospho-Ser121) antibody.