• Catalog Number:

  E011045-1

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human STAT3 around the phosphorylation site of tyrosine 705 (A-P-YP-L-K).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P40763

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100

• Specificity Sensitivity:

  STAT3 (phospho-Tyr705) antibody detects endogenous levels of STAT3 only when phosphorylated at tyrosine 705.

• References:

  Fan G, et al. (2003) J Biol Chem. 278(52): 52432-52436. Barry FA, et al. (2003) FEBS Lett. 553(1-2): 173-178. Welsh, et al. (1996) Trends Cell Biol. 6: 274-279. Srivastava A K, et al. (1998) Mol Cell Biochem. 182: 135-141.

P-Peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using STAT3 (phospho-Tyr705) antibody (E011045).

Western blot analysis of extracts from HeLa cells using STAT3 (phospho-Tyr705) antibody (E011045).