• Catalog Number:

  E011152-1

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human IκB-α around the phosphorylation site of serine 32/36 (H-D-SP-G-L-D-SP -M-K).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P25963

• Applications:

  IHC: 1:50~1:100

• Specificity Sensitivity:

  IκB-α (phospho-Ser32/Ser36) antibody detects endogenous levels of IκB-α only when phosphorylated at serine 32/36.

• References:

  Shieh, S. Y. et al. (1999) EMBO J. 18, 1815-1823. Honda, R. et al. (1997) FEBS Lett. 420, 25-27. Hirao, A. et al. (2000) Science 287, 1824-1827. Tibbetts, R. S. et al. (1999) Genes Dev. 13, 152-157. Chehab,N.H.et al. (1999) Proc .Natl .Acad. Sci. USA 96, 13777-13782.

P-Peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using IκB-α (phospho-Ser32/Ser36) antibody (E011152).