• Catalog Number:

  E011038-2

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Elk-1 around the phosphorylation site of threonine 417 (L-S-TP-P-V).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P19419

• Applications:

  IHC: 1:50~1:100 IF:1:100~1:200

• Specificity Sensitivity:

  Elk-1 (phospho-Thr417) antibody detects endogenous levels of Elk-1 only when phosphorylated at threonine 417.

• References:

  Huang Y, et al. (2000) Biochem Biophys Res Commun. 271(2): 456-463. Marais R, et al. (1993) Cell 73:381-393. Kortenjann M, et al. (1994) Mol Cell Biol. 14:4815-4824. Hill C S, et al. (1995) Cell. 80:199-211. Cavigelli M, et al. (1995) EMBO J. 14:5957-5964.

P-Peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Elk-1 (phospho-Thr417) antibody (E011038).

Immunofluorescence staining of methanol-fixed HeLa cells using Elk-1 (phospho-Thr417) antibody (E011038, Red).