• Catalog Number:

  E20-60698

• Amount:

  100ul

• Form of Antibody:

  Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. .

• Storage/Stability:

  Stability: -20°C/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized peptide derived from human p130 Cas around the phosphorylation site of Tyr249. AA range215-264

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Reactivity:

  Human,Mouse,Rat

• Source:

  Rabbit

• Applications:

  WB:1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.

• Other Names:

  BCAR1; CAS; CASS1; CRKAS; Breast cancer anti-estrogen resistance protein 1; CRK-associated substrate; Cas scaffolding protein family member 1; p130cas

• Specificity Sensitivity:

  Phospho-p130 Cas (Y249) Polyclonal Antibody detects endogenous levels of p130 Cas protein only when phosphorylated at Y249.

Enzyme-Linked Immunosorbent Assay (Phospho- ELISA) for Immunogen Phosphopeptide (Phospho- left) and Non-Phosphopeptide (Phospho-right), using p130 Cas (Phospho-Tyr249) Antibody

WB analysis of lysates from HepG2 cells treated with EGF 200ng/ml 30' and A549 cells treated with PMA 125ng/ml 30', using p130 Cas (Phospho- Tyr249) Antibody. The lane on the right is blocked with the phospho peptide.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using p130 Cas (Phospho- Tyr249) Antibody. The picture on the right is blocked with the phospho peptide.