• Catalog Number:

  E011035-2

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Myc around the phosphorylation site of threonine 358 (R-R-TP-H-N).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P01106

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 IF:1:100~1:200

• Specificity Sensitivity:

  Myc (phospho-Thr358) antibody detects endogenous levels of Myc only when phosphorylated at threonine 358.

• References:

  Baudino T A, et al. (2001) Mol Cell Biol. 21: 691-702. Blackwood E M, et al. (1991) Science. 251:1211-1217. Henriksson M, et al. (1996) Adv Cancer Res. 68: 109-182. Grandori C, et al. (2000) Annu Rev Cell Dev Biol. 16: 653-699.

P-Peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Myc (phospho- Thr358) antibody (E011035).

Western blot analysis of extracts from HT-29 cells treated with UV (20min), using Myc (Ab-358) antibody (E021035, Lane 1 and 2) and Myc (phospho-Thr358) antibody (E011035, Lane 3 and 4).