• Catalog Number:

  E1A7123A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human IRS-1 around the phosphorylation site of serine 639 (P-K-SP-V-S).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P35568

• Applications:

  WB: 1:500~1:3000 IHC: 1:50~1:100 ELISA: 1:20000

• Alter Name:

  IRS-1, IRS1, Insulin receptor substrate-1

• Specificity Sensitivity:

  IRS-1 (phospho-Ser639) antibody detects endogenous levels of IRS-1 only when phosphorylated at serine 639.

• References:

  Ozes ON, et al. (2001) Proc Natl Acad Sci USA; 98(8): 4640-4645. Tzatsos A, et al. (2006) Mol Cell Biol; 26(1): 63-76. Steppan CM, et al. (2005) Mol Cell Biol; 25(4): 1569-1575. Batty IH, et al. (2004) Biochem J; 379(Pt 3): 641-651.

• All Sites:

  Human: Ser639; Mouse: Ser 635; Rat: Ser 635

P-peptide - +

Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue, using IRS-1 (phospho-Ser639) antibody. Western blot analysis of extracts from HepG2 cells, using IRS-1 (phospho-Ser639) antibody.