Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human JunD around the phosphorylation site of serine 255 (G-E-SP-P-P).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P17535
Applications:
WB: 1:500~1:1000 IHC: 1:50~1:100
Specificity Sensitivity:
JunD (phospho-Ser255) antibody detects endogenous levels of JunD only when phosphorylated at serine 255.
References:
Beausoleil S A, et al. (2004) Proc Natl Acad Sci U S A. 101(33): 12130-12135.
P-Peptide - +
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using JunD (phospho- Ser255) antibody (E011028).
Western blot analysis of extracts from 293 cells using JunD (phospho-Ser255) antibody (E011028).