• Catalog Number:

  E1A7042B-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized non-phosphopeptide derived from human Chk1 around the phosphorylation site of serine 345 (S-F-SP-Q-P).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  O14757

• Applications:

  WB: 1:500~1:1000 ELISA: 1:40000

• Alter Name:

  CHEK1, Chk1, kinase Chk1

• Specificity Sensitivity:

  Chk1 (Ab-345) antibody detects endogenous levels of total Chk1 protein.

• References:

  Martinho, R.G. et al. (1998) EMBO J. 17, 7239-7249. Zhao, H. et al. (2001) Mol. Cell. Biol. 21, 4129-4139. Zeng, Y. et al. (1998) Nature 395, 507-510. Shieh, S. et al. (2000) Genes Dev. 14, 289-300.

Western blot analysis of extracts from HT29 cells untreated or treated with UV, using Chk1 (Ab-345) antibody (#B7042, Line1 and 2)