• Catalog Number:

  E1A0882A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Cytokeratin 18 around the phosphorylation site of serine 52 (S-T-SP-F-R).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human

• SwissProtNo:

  P05783

• Applications:

  WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:10000

• Other Names:

  CK 18, CK18, CYK18, Cytokeratin endo B, K18, K1C18, KERD, KRT1-18, KRT18, Keratin, Keratin D, Keratin, type I cytoskeletal 18, cytokeratin 18, type I cytoskeletal 18

• Specificity Sensitivity:

  Cytokeratin 18 (Phospho-Ser52) antibody detects endogenous levels of Cytokeratin 18 only when phosphorylated at serine 52.

• References:

  Oshima R.G., Differentiation 33:61-68(1986). Ji H., Electrophoresis 18:605-613(1997). Ku N.-O., J. Biol. Chem. 270:11820-11827(1995).

• All Sites:

  Human: Ser52

P-peptide - + Western blot analysis of extracts from HepG2 cells, using Cytokeratin 18 (Phospho-Ser52) antibody. Immunofluorescence analysis of HeLa cells, using Cytokeratin 18 (Phospho-Ser52) antibody.