• Catalog Number:

  E1A0635A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Cyclin B1 around the phosphorylation site of serine 147 (A-F-SP-D-V).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse

• SwissProtNo:

  P14635

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000

• Other Names:

  CCN-2, CCNB, CCNB1, CCNB1-RS1, CYCB, CYCB1, G2/mitotic-specific cyclin B1

• Specificity Sensitivity:

  Cyclin B1 (Phospho-Ser147) antibody detects endogenous levels of Cyclin B1 only when phosphorylated at serine 147.

• References:

  Catherine A. Rugg, AACR Meeting Abstracts, Apr 2005; 2005: 1047. Jianping Gong, Cancer Res., Aug 1994; 54: 4285 - 4288. Lourdes A. Gomez, Mol. Cancer Ther., May 2007; 6: 1534 - 1543. Clare E. Davy, J. Virol., Apr 2005; 79: 3998 - 4011.

• All Sites:

  Human: Ser147; Mouse: Ser144

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human placenta tissue using Cyclin B1 (Phospho-Ser147) antibody. Western blot analysis of extracts from Jurkat cells, treated with UV (15mins), using Cyclin B1 (Phospho-Ser147) antibody.