• Catalog Number:

  E1A0696A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human p130 Cas around the phosphorylation site of tyrosine 249 (D-I-YP-D-V).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P56945

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000

• Other Names:

  BCA1, BCAR1, Breast cancer anti-estrogen resistance 1 protein, CAS, CRK-associated substrate, CRKAS

• Specificity Sensitivity:

  p130 Cas (Phospho-Tyr249) antibody detects endogenous levels of p130 Cas only when phosphorylated at tyrosine 249.

• References:

  Brinkman A., J. Natl. Cancer Inst. 92:112-120(2000). Ota T., Nat. Genet. 36:40-45(2004). Amanchy R., J. Proteome Res. 4:1661-1671(2005).

• All Sites:

  Human: Tyr249; Mouse: Tyr253; Rat: Tyr347

Western blot analysis of extracts from HepG2 cells treated with EGF (200ng/ml, 30mins) and A549 cells treated with PMA (125ng/ml, 30mins), using p130 Cas (Phospho-Tyr249) antibody.