• Catalog Number:

  E1A0550A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human PLB around the phosphorylation site of serine 16 and threonine 17 (R-A-SP-TP-I-E).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P26678

• Applications:

  IF: 1:500~1:1000 ELISA: 1:1000

• Other Names:

  PLN, PPLA, PPLA_DOG, cardiac phospholamban, phospholamban

• Specificity Sensitivity:

  PLB (Phospho-Ser16+Thr17) antibody detects endogenous levels of PLB only when phosphorylated at serine 16 and threonine 17.

• References:

  Fujii J., J. Biol. Chem. 266:11669-11675(1991). McTiernan C.F., J. Mol. Cell. Cardiol. 31:679-692(1999). Haghighi K., Proc. Natl. Acad. Sci. U.S.A. 103:1388-1393(2006).

• All Sites:

  Human: Ser16+Thr17; Mouse: Ser16+Thr17; Rat: Ser16+Thr17

P-peptide - + Immunofluorescence analysis of HuvEc cells, using PLB (Phospho-Ser16+Thr17) antibody.