• Catalog Number:

  E1A0607A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Akt around the phosphorylation site of tyrosine 474 (F-S-YP-S-A).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P31749

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000

• Other Names:

  AKT, AKT1 kinase, C-AKT, EC 2.7.11.1, PKB, PKB-alpha, Protein kinase B, RAC, RAC-PK-alpha, RAC-alpha serine/threonine kinase, kinase Akt1

• Specificity Sensitivity:

  Akt (Phospho-Tyr474) antibody detects endogenous levels of Akt only when phosphorylated at tyrosine 474.

• References:

  Kirsten H. Limesand, Mol. Cell. Biol., Dec 2006; 26: 8840 - 8856. Brian T. O’Neill, J. Clin. Invest., Aug 2005; 115: 2059 - 2064. Zhong-Zhou Yang, J. Biol. Chem., Aug 2003; 278: 32124 - 32131. -------------------------------------------------------------------------------------------------------------------------------------------------------- --------

• All Sites:

  Human: Tyr474; Mouse: Tyr474; Rat: Tyr474

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human ovary tissue using Akt (Phospho-Tyr474) antibody. Western blot analysis of extracts from COS7cells, treated with UV (15mins), using Akt (Phospho-Tyr474) antibody.