• Catalog Number:

  E1A0088B-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized non-phosphopeptide derived from human SRF around the phosphorylation site of serine 99 (S-L-S-E-M).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P11831

• Applications:

  WB: 1:500~1000 IHC: 1:50~100 ELISA: 1:5000

• Alter Name:

  serum response factor

• Specificity Sensitivity:

  SRF (Ab-99) antibody detects endogenous levels of total SRF protein.

• References:

  SH Liu, J. Biol. Chem., Oct 1993; 268: 21147 - 21154. Aurore L’honore, Mol. Biol. Cell, Mar 2007; 10.1091/mbc.E06-09-0867. Olaf Heidenreich, J. Biol. Chem., May 1999; 274: 14434. Isabella Irrcher and David A. Hood, J Appl Physiol, Dec 2004; 97: 2207 - 2213.

Peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using SRF (Ab-99) antibody (#B0088).

Western blot analysis of extracts from NIH/3T3 cells treated with PMA (125ng/ml, 30mins), using SRF (Ab-99) antibody (#B0088).