• Catalog Number:

  E1A0440A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human IKK-α/β around the phosphorylation site of serine 180/181 (C-T-SP-F-V).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse

• SwissProtNo:

  O15111/O14920

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000

• Other Names:

  CHUK, Conserved helix-loop-helix ubiquitous kinase, I kappa-B kinase alpha, I-kappa-B kinase 1, IKK-A, IKK-alpha, IKK1, IKKA, IkBKA, IkappaB kinase, Inhibitor of nuclear factor kappa-B kinase alpha subunit, NFKBIKA, Nuclear factor NFkappaB inhibitor kinase alpha, kinase IKK-alpha, EC 2.7.11.10, I-kappa-B kinase 2, I-kappa-B-kinase beta, IKK-B, IKK-beta, IKK2, IKKB, IkBKB, NFKBIKB, Nuclear factor NF-kappa-B inhibitor kinase beta, kinase IKK-beta

• Specificity Sensitivity:

  IKK-α/β (Phospho-Ser180/181) antibody detects endogenous levels of IKK-α/β only when phosphorylated at serine 180/181.

• References:

  Tsuyoshi Shuto, PNAS, Jul 2001; 98: 8774. Bing-Chang Chen, J. Immunol., Oct 2004; 173: 5219 - 5228. Jennifer Harris, J. Immunol., Aug 2006; 177: 2527 - 2535.

• All Sites:

  Human: Ser180; Mouse: Ser180

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human heart tissue using IKK-α/β (Phospho-Ser180/181) antibody. Western blot analysis of extracts from HepG2 cells, treated with TNF (20ng/ml, 5mins), using IKK-α/β (Phospho-Ser180/181) antibody.