• Catalog Number:

  E021260-1

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized non-phosphopeptide derived from human Histone H2A.X around the phosphorylation site of serine 139 (Q-A-SP-Q-E).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P16104

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 IF:1:100~1:200

• Specificity Sensitivity:

  Histone H2A.X (Ab-139) antibody detects endogenous levels of total Histone H2A.X protein.

• References:

  Yaneva M, et al. (2005) Nucleic Acids Res. 33(16): 5320-5330. Tsukuda T, et al.(2006) Nature. Author manuscript; available in PMC 2006 March 6

Peptide - + Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using Histone H2A.X (Ab-139) antibody (E021260).

Western blot analysis of extract from 293 cells untreated or treated with UV, using Histone H2A.X (Ab-139) antibody (E021260, Lane 1 and 2) and Histone H2A.X (phospho- Ser139) antibody (E011268, Lane 3 and 4).