• Catalog Number:

  E1A8537A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human C-RAF around the phosphorylation site of serine 642 (T-T-SP-P-R).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%);Mouse (Identities = 100%, Positives = 100%); Rat (Identities = 100%, Positives = 100%)

• SwissProtNo:

  P04049

• Applications:

  WB: 1:500~1:1000 ELISA: 1:40000

• Other Names:

  C-RAF; C-Raf; CRAF; kinase Raf1; Protein kinase raf 1; RAF proto-oncogene serine/threonine-protein kinase; Raf proto-oncogene serine/threonine-protein kinase; RAF-1; Raf-1; RAF1; Similar to murine leukemia viral (V-raf-1) oncogene homolog 1

• Specificity Sensitivity:

  C-RAF (Phospho-Ser642) antibody detects endogenous levels of C-RAF only when phosphorylated at serine 642.

• References:

  Bonner T.I., Nucleic Acids Res. 14:1009-1015(1986). Ota T., Nat. Genet. 36:40-45(2004). Mural R.J., Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.

• All Sites:

  Human: Ser642; Mouse: Ser642; Rat: Ser642

Western blot analysis of extracts from HuvEc cells and K562 cells treated with PMA, using C-RAF (Phospho-Ser642) antibody.