Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human NMDAR1 around the phosphorylation site of serine 897 (R-S-SP-K-D).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
Q05586
Applications:
WB: 1:500~1:1000 IHC: 1:50~1:100
Specificity Sensitivity:
NMDAR1 (Phospho-Ser897) antibody detects endogenous levels of NMDAR1 only when phosphorylated at serine 897.
References:
Emamian ES, Karayiorgou M, Gogos JA (2004) J Neurosci 24, 1561-4.
Chan SF, Sucher NJ (2001) J Neurosci 21, 7985-92.
Liu, X.B. et al. (2004) J. Neurosci. 24, 8885-8895.
Westphal, R.S. et al. (1999) Science 285, 93-96.
Tingley, W.G. et al. (1997) J. Biol. Chem. 272, 5157-5166.
Raman, I.M. et al. (1996) Neuron 16, 415-421.
Immunohistochemical analysis of paraffin-embedded human brain tissue using NMDAR1 (Phospho-Ser897) Antibody. (E012098).