• Catalog Number:

  E1A8481A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human YB1 around the phosphorylation site of serine 102 (L-R-SP-V-G).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%)

• SwissProtNo:

  P67809

• Applications:

  WB: 1:500~1:1000 ELISA: 1:40000

• Other Names:

  CBF-A; CCAAT-binding transcription factor I subunit A; DBPB; DNA-binding protein B; EFI-A; Enhancer factor I subunit A; MSY-1; NSEP1; Nuclease sensitive element binding protein 1; Y box binding protein-1; Y-box transcription factor; YB1; YBOX1; YBX1

• Specificity Sensitivity:

  YB1 (Phospho-Ser102) antibody detects endogenous levels of YB1 only when phosphorylated at serine 102.

• References:

  Sakura H., Gene 73:499-507(1988). Didier D.K. Proc. Natl. Acad. Sci. U.S.A. 85:7322-7326(1988). Kolluri R., Nucleic Acids Res. 19:4771-4771(1991).

• All Sites:

  Human: Ser102; Mouse: Ser100; Rat: Ser100

Western blot analysis of extracts from HepG2 cells, treated with PMA (125ng/mll, 15mins), using YB1 (Phospho-Ser102) antibody.