• Catalog Number:

  E1A8317A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Claudin 3 around the phosphorylation site of tyrosine 219 (R-K-D-YP-V).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%)

• SwissProtNo:

  O15551

• Applications:

  WB: 1:500~1:1000 ELISA: 1:1000

• Other Names:

  CLD3; CLDN3; Clostridium perfringens enterotoxin receptor 2; CPE-R 2; CPE-receptor 2; HRVP1; Ventral prostate 1 protein homolog

• Specificity Sensitivity:

  Claudin 3 (Phospho-Tyr219) antibody detects endogenous levels of Claudin 3 only when phosphorylated at tyrosine 219.

• References:

  Peacock R.E., Genomics 46:443-449(1997). Katahira J., J. Biol. Chem. 272:26652-26658(1997). The MGC Project Team; Genome Res. 14:2121-2127(2004).

• All Sites:

  Human: Tyr219

Western blot analysis of extracts from COLO cells, treated with EGF (200ng/ml, 30mins), using Claudin 3 (Phospho-Tyr219) antibody.