• Catalog Number:

  E1A8476A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human SIRT1 around the phosphorylation site of serine47.

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human; Mouse

• SwissProtNo:

  Q96EB6

• Applications:

  WB: 1:500~1:1000 ELISA: 1:10000

• Other Names:

  hSIR2; hSIRT1; NAD-dependent deacetylase sirtuin-1; sir2-like 1; SIR2-like protein 1; SIR2alpha; SIR2L1; SIRT1; sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae); sirtuin 1; sirtuin type 1

• Specificity Sensitivity:

  SirT1 (Phospho-Ser47) antibody detects endogenous levels of SIRT1 only when phosphorylated at serine47.

• References:

  Takata T.,Biochem. Biophys. Res. Commun. 301:250-257(2003). Deloukas P., Nature 429:375-381(2004). Deloukas P., Nature 429:375-381(2004).

• All Sites:

  Human: Ser47; Mouse: Ser46

Western blot analysis of extracts from JK cells, using SirT1 (Phospho-Ser47)antibody.