• Catalog Number:

  E1A1053A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human IκB-α around the phosphorylation site of tyrosine 305 (L-P-YP-D-D).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%)

• SwissProtNo:

  P25963

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000

• Other Names:

  I-kappa-B-alpha; IkappaBalpha; IKBA; MAD3; Major histocompatibility complex enhancer-binding protein MAD3; NF-kappaB inhibitor alpha; NFKBI; NFKBIA; RL/IF-1

• Specificity Sensitivity:

  IκB-α (Phospho-Tyr305) antibody detects endogenous levels of IκB-α only when phosphorylated at tyrosine 305.

• References:

  Haskill S., Cell 65:1281-1289(1991). Jungnickel B., J. Exp. Med. 191:395-402(2000). Ganchi P.A., Mol. Biol. Cell 3:1339-1352(1992).

• All Sites:

  Human: Tyr305; Mouse: Tyr302; Rat: Tyr302

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human lymph node tissue using IκB-α (Phospho-Tyr305) antibody. Western blot analysis of extracts from COS7 cells, treated with nocodazole (1ug/ml, 16hours), using IκB-α (Phospho-Tyr305) antibody.