• Catalog Number:

  E1A8460A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human HER3 around the phosphorylation site of tyrosine 1197 (E-E-YP-E-Y).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%)

• SwissProtNo:

  P21860

• Applications:

  WB: 1:500~1:1000 ELISA: 1:40000

• Other Names:

  c-erbB3; EC 2.7.10.1; ERB3; ERBB3; HER3; kinase ErbB3; Receptor protein-tyrosine kinase erbB-3 precursor; Tyrosine kinase-type cell surface receptor HER3

• Specificity Sensitivity:

  HER3 (Phospho-Tyr1197) antibody detects endogenous levels of HER3 only when phosphorylated at tyrosine 1197.

• References:

  Kraus M.H., Proc. Natl. Acad. Sci. U.S.A. 86:9193-9197(1989). Plowman G.D., Proc. Natl. Acad. Sci. U.S.A. 87:4905-4909(1990). Katoh M., Biochem. Biophys. Res. Commun. 192:1189-1197(1993).

• All Sites:

  Human: Tyr1197; Mouse: Tyr1194; Rat: Tyr1194

Western blot analysis of extracts from HepG2 cells, using HER3 (Phospho-Tyr1197) antibody. HER3 (Phospho-Tyr1197) antibody reacts with epitope-specific phosphopeptide and corresponding non-phosphopeptide. The absorbance readings at 450 nM are shown in the ELISA figure.