• Catalog Number:

  E1A7117B-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized non-phosphopeptide derived from human IKKα around the phosphorylation site of Threonine 23.

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  O15111

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000

• Specificity Sensitivity:

  IKKα (Ab-23) Antibody detects endogenous levels of total IKKα protein.

• References:

  Yuan ZQ, et al.(2002)J Biol Chem; 277(33): 29973-82. Ozes ON, et al. (1999)Nature; 401(6748): 82-5.

Immunohistochemical analysis of paraffin-embedded colon carcinoma, using IKKα (Ab-23) Antibody (#B7117). Left: Untreated; Right: Treated with synthesized peptide.

Western blot analysis of extracts using IKKα (Ab-23) Antibody (#B7117). Line1:The extracts from 293 cells treated with EGF; Line2: The extracts from MDA-MB-435 cells treated with EGF; Line3: The extracts from MDA-MB-435 cells untreated.