• Catalog Number:

  E1A8443A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human TR-β1 around the phosphorylation site of serine 142 (H-P-SP-Y-S).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%)

• SwissProtNo:

  P10828

• Applications:

  WB: 1:500~1:1000 ELISA: 1:5000

• Other Names:

  C-ERBA-BETA; ERBA2; NR1A2; THB; THB1; THR1; THRB; Thyroid hormone receptor beta-1

• Specificity Sensitivity:

  TR-β1 (Phospho-Ser142) antibody detects endogenous levels of TR-β1 only when phosphorylated at serine 142.

• References:

  Weinberger C., Nature 324-641-646(1986). Weinberger C., Cold Spring Harb. Symp. Quant. Biol. 51:759-772(1988). Sakurai A., Mol. Cell. Endocrinol. 71:83-91(1990).

• All Sites:

  Human: Ser142; Mouse: Ser142; Rat: Ser142

Western blot analysis of extracts from 293 cells, treated with PMA (125ng/ml, 30mins), using TR-β1 (Phospho-Ser142) antibody.