• Catalog Number:

  E1A1073A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human IRAK1 around the phosphorylation site of threonine 100 (I-I-TP-A-W).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse

• SwissProtNo:

  P51617

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000

• Other Names:

  EC 2.7.11.1, IL1RAK, IRAK, IRAK-1, Interleukin-1 receptor-associated kinase 1, Pelle-like protein kinase, kinase IRAK1, mPLK

• Specificity Sensitivity:

  IRAK1 (Phospho-Thr100) antibody detects endogenous levels of IRAK1 only when phosphorylated at threonine 100.

• References:

  Cao Z., Science 271:1128-1131(1996). Reichwald K., Mamm. Genome 11:182-190(2000). Jensen L.E., J. Biol. Chem. 276:29037-29044(2001).

• All Sites:

  Human: Thr100; Mouse: Thr100

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using IRAK1 (Phospho-Thr100) antibody. Western blot analysis of extracts from Jurkat cells, treated with heat shock, using IRAK1 (Phospho-Thr100) antibody.