• Catalog Number:

  E1A8400A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human PLD2 around the phosphorylation site of tyrosine 169 (E-N-YP-L-N).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%)

• SwissProtNo:

  O14939

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000

• Other Names:

  choline phosphatase 2; EC 3.1.4.4; phosphatidylcholine-hydrolyzing phospholipase D2; phospholipase D2; PLD 2; PLD1C

• Specificity Sensitivity:

  PLD2 (Phospho-Tyr169) antibody detects endogenous levels of PLD2 only when phosphorylated at tyrosine 169.

• References:

  Steed P.M., FASEB J. 12:1309-1317(1998). Lopez I., J. Biol. Chem. 273:12846-12852(1998). Divecha N., EMBO J. 19:5440-5449(2000).

• All Sites:

  Human: Tyr169; Mouse: Tyr169; Rat: Tyr169

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human brain tissue using PLD2 (Phospho-Tyr169) antibody. Western blot analysis of extracts from Jurkat cells, treated with TNF (20ng/ml, 30mins), using PLD2 (Phospho-Tyr169) antibody.