• Catalog Number:

  E1A8075A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human AKT1/2/3 around the phosphorylation site of tyrosine 315/316/312 (P-E-YP-L-A).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%)

• SwissProtNo:

  P31749/P31751/Q9Y243

• Applications:

  IHC: 1:50~1:100 ELISA: 1:20000

• Other Names:

  AKT; AKT1 kinase; C-AKT; EC 2.7.11.1; kinase Akt1; PKB; PKB-alpha; Protein kinase B; RAC; RAC-alpha serine/threonine kinase; RAC-PK-alpha

• Specificity Sensitivity:

  AKT1/2/3 (Phospho-Tyr315/316/312) antibody detects endogenous levels of AKT1/2/3 only when phosphorylated at tyrosine 315/316/312.

• References:

  Jones P.F., Proc. Natl. Acad. Sci. U.S.A. 88:4171-4175(1991). Matsubara A., Diabetologia 44:910-913(2001). The MGC Project Team, Genome Res. 14:2121-2127(2004).

• All Sites:

  Human: Tyr315/316/312; Mouse: Tyr315/316/312; Rat: Tyr315/316/312

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using AKT1/2/3 (Phospho-Tyr315/316/312) antibody. AKT1/2/3 (Phospho-Tyr315/316/312) antibody reacts with epitope-specific phosphopeptide and corresponding non-phosphopeptide. The absorbance readings at 450 nM are shown in the ELISA figure.