• Catalog Number:

  E1A8339A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human E2A around the phosphorylation site of threonine 355 (P-S-TP-P-V).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%)

• SwissProtNo:

  P15923

• Applications:

  IHC: 1:50~1:100 ELISA: 1:1000

• Other Names:

  E12; Immunoglobulin enhancer binding factor E12/E47; Immunoglobulin transcription factor-1; ITF1; Kappa-E2-binding factor; TCF-3; TCF3; TFE2; transcription factor 3 (E2A immunoglobulin enhancer binding factors E12/E47); Transcription factor E2-alpha; Transcription factor ITF-1; Transcription factor-3

• Specificity Sensitivity:

  E2A (Phospho-Thr355) antibody detects endogenous levels of E2A only when phosphorylated at threonine 355.

• References:

  Kamps M.P., Cell 60:547-555(1990). Nourse J., Cell 60:535-545(1990). Grimwood J., Nature 428:529-535(2004).

• All Sites:

  Human: Thr355; Mouse: Thr353; Rat: Thr351

P-peptide - + Immunohistochemistry analysis of paraffin-embedded human brain tissue using E2A (Phospho-Thr355) antibody.