• Catalog Number:

  E011255-2

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human RelB around the phosphorylation site of serine 552 (L-L-SP-P-G).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  Q01201

• Applications:

  WB: 1:500~1:1000 IHC: 1:50-1:100

• Specificity Sensitivity:

  RelB (phospho-Ser552) antibody detects endogenous levels of RelB only when phosphorylated at serine 552.

• References:

  Marienfeld R, et al. (2001) Oncogene. 20 (56): 8142-7. Charlotte S. Kaetzel1, et al. (2005) Immunological Reviews Volume 206: 83  Elwira Pyz, et al. (2006) J Immunol. 176:7447-55

P-Peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using RelB (phospho- Ser552) antibody (E011255).

Western blot analysis of extracts from A431 cells, untreated or treated with EGF (200ng/ml 10min), using RelB (Ab-552) antibody (E021247, Line 1 and 2) and RelB (phospho- Ser552) antibody (E011255, Line 3 and 4).