• Catalog Number:

  E011504-2

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human JNK1/JNK2 around the phosphorylation site of Thr183/Tyr185(M-M-TP-P-YP - V - V ).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P45983 P45984 P53779

• Applications:

  WB:1:500-1000 IF:1:100~1:200

• Specificity Sensitivity:

  JNK1/JNK2 (phospho-Thr183/Tyr185) Antibody detects endogenous levels of JNK1/JNK2 only when phosphorylated at Thr183/Tyr185.

• References:

  Davis, R.J. (1999) Biochem Soc Symp 64, 1-12. Ichijo, H. (1999) Oncogene 18, 6087-93. Kyriakis, J.M. and Avruch, J. (2001) Physiol Rev 81, 807-69.

Immunofluorescence staining of methanol-fixed HeLa cells using JNK1/JNK2 (phospho-Thr183/Tyr185) Antibody (E011504, Red).

Anisomycin + + p-peptide - + Western blot analysis of extract from C6 cells (Rat) using JNK1/JNK2 (phospho-Thr183/Tyr185) Antibody (E011504).