Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human CDC25C around the phosphorylation site of serine 198 (E-F-SP-L-K).
Purification:
The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using
epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
CDC25C (Phospho-Ser198) antibody detects endogenous levels of CDC25C only when phosphorylated at serine 198.
References:
Sadhu K., Proc. Natl. Acad. Sci. U.S.A. 87:5139-5143(1990).
Bureik M., Int. J. Oncol. 17:1251-1258(2000).
Wegener S., Eur. J. Cell Biol. 79:810-815(2000).
All Sites:
Human: Ser198
P-peptide - +
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using CDC25C (Phospho-Ser198) antibody.