• Catalog Number:

  E1A8320A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Claudin 6 around the phosphorylation site of tyrosine 219 (T-K-N-YP-V).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody againstnon-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%)

• SwissProtNo:

  P56747

• Applications:

  WB: 1:500~1:1000 ELISA: 1:1000

• Other Names:

  claudin 6; claudin-6; CLD6; Skullin 2

• Specificity Sensitivity:

  Claudin 6 (Phospho-Tyr219) antibody detects endogenous levels of Claudin 6 only when phosphorylated at tyrosine 219.

• References:

  Keen T.J., Submitted (SEP-1999) to the EMBL/GenBank/DDBJ databases. Troy T., Submitted (FEB-2001) to the EMBL/GenBank/DDBJ databases. Clark H.F., Genome Res. 13:2265-2270(2003).

• All Sites:

  Human: Tyr219; Mouse: Tyr218

Western blot analysis of extracts from RAW264.7 cells, treated with UV (5mins), using Claudin 6 (Phospho-Tyr219) antibody.