• Catalog Number:

  E1A8308A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human CAD around the phosphorylation site of threonine 456 (P-I-TP-P-H).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 91%, Positives = 100%)

• SwissProtNo:

  P27708

• Applications:

  IHC: 1:50~1:100 ELISA: 1:5000

• Other Names:

  CAD protein; EC 2.1.3.2; EC 3.5.2.3; EC 6.3.5.5; glutamine-dependent carbamoyl-phosphate synthase; PYR1

• Specificity Sensitivity:

  CAD (Phospho-Thr456) antibody detects endogenous levels of CAD only when phosphorylated at threonine 456.

• References:

  Iwahana H., Biochem. Biophys. Res. Commun. 219:249-255(1996). Davidson J.N., DNA Cell Biol. 9:667-676(1990). Olsen J.V., Cell 127:635-648(2006).

• All Sites:

  Human: Thr456; Mouse: Thr456

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using CAD (Phospho-Thr456) antibody. CAD (Phospho-Thr456) antibody reacts with epitope-specific phosphopeptide and corresponding non-phosphopeptide. The absorbance readings at 450 nM are shown in the ELISA figure.