Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human LAT around the phosphorylation site of tyrosine 161 (P-G-YP-L-V).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human (Identities = 100%, Positives = 100%)
SwissProtNo:
O43561
Applications:
WB: 1:500~1:1000 ELISA: 1:10000
Other Names:
36 kDa phospho-tyrosine adaptor protein; Linker for activation of T cells; p36-38; pp36
Specificity Sensitivity:
LAT (Ab-161) antibody detects endogenous levels of total LAT protein.
References:
Zhang W., Cell 92:83-92(1998).
Weber J.R., J. Exp. Med. 187:1157-1161(1998).
Zhang W., Immunity 9:239-246(1998).
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Western blot analysis of extracts from Jurkat cells, using LAT (Ab-161) antibody.