• Catalog Number:

  E1A8306A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human BRCA1 around the phosphorylation site of serine 1457 (L-T-SP-Q-K).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%)

• SwissProtNo:

  P38398

• Applications:

  WB: 1:500~1:1000 ELISA: 1:1000

• Other Names:

  BRCA1; Breast cancer type 1 susceptibility protein; familial breast/ovarian cancer gene 1

• Specificity Sensitivity:

  BRCA1 (Phospho-Ser1457) antibody detects endogenous levels of BRCA1 only when phosphorylated at serine 1457.

• References:

  Miki Y., Science 266:66-71(1994). Smith T.M., Genome Res. 6:1029-1049(1996). Wilson C.A., Oncogene 14:1-16(1997).

• All Sites:

  Human: Ser1457

Western blot analysis of extracts from 293 cells, treated with epo (20U/ml, 15mins), using BRCA1 (Phospho-Ser1457) antibody.