• Catalog Number:

  E1A7100A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human HDAC4 around the phosphorylation site of serine 632 (A-Q-SP-S-P).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P56524

• Applications:

  WB: 1:500~1:1000 ELISA: 1:20000

• Specificity Sensitivity:

  HDAC4 (phospho-Ser632) antibody detects endogenous levels of HDAC4 only when phosphorylated at serine 632.

• References:

  Wang AH, et al. (2000) Mol Cell Biol. 20(18): 6904-6912. Grozinger CM, et al. (2000) Proc Natl Acad Sci U S A. 97(14): 7835-7840.

Calyculin A - - - + Peptide - + - - Western blot analysis of extracts from Jurkat cells using HDAC4 (Ab-632) antibody (Line 1 and 2) and HDAC4 (phospho-Ser632) antibody (Line 3 and 4).