• Catalog Number:

  E1A8299A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Bcl-6 around the phosphorylation site of serine 333 (P-Q-SP-P-Q).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%)

• SwissProtNo:

  P41182

• Applications:

  WB: 1:500~1:1000 ELISA: 1:1000

• Other Names:

  B-cell CLL/lymphoma 6; B-cell lymphoma 6 protein; B-cell lymphoma 6 protein homolog; Bcl-5; Bcl5; Bcl6; LAZ-3 protein; LAZ3; Zinc finger protein 51; ZNF51

• Specificity Sensitivity:

  Bcl-6 (Phospho-Ser333) antibody detects endogenous levels of Bcl-6 only when phosphorylated at serine 333.

• References:

  Kerckaert J.-P., Nat. Genet. 5:66-70(1993). Ye B.H., Science 262:747-750(1993). Miki T., Blood 83:26-32(1994). ----------------------------------------------------------------------------

• All Sites:

  Human: Ser333; Mouse: Ser334; Rat: Ser334

Western blot analysis of extracts from COLO cells, treated with insulin (0.01U/ml, 15mins), using Bcl-6 (Phospho-Ser333) antibody.