• Catalog Number:

  E1A7041A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Chk1 around the phosphorylation site of Serine 317.

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Rat

• SwissProtNo:

  O14757

• Applications:

  WB: 1:500~1:1000 ELISA: 1:5000

• Specificity Sensitivity:

  Chk1 (Phospho-Ser317) Antibody detects endogenous levels of Chk1 only when phosphorylated at Serine 317.

• References:

  Zhang YW, et al. (2005) Mol Cell; 19(5): 607-18. Bhoumik A, et al. (2005) Mol Cell; 18(5): 577-87. Rocha S, et al. (2005) EMBO J. Clarke CA, et al. (2005) Biochem J. Yu X, et al. (2004) Mol Cell Biol; 24(21): 9478-86.

Western blot analysis of extracts using Chk1 (Ab-317) Antibody. Line1: The extracts from MCF7 cells; Line2: The extracts from COLO cells.