• Catalog Number:

  E1A8297A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Bax around the phosphorylation site of serine 184 (T-A-SP-L-T).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%)

• SwissProtNo:

  Q07812

• Applications:

  IHC: 1:50~1:100 ELISA: 1:10000

• Other Names:

  Apoptosis regulator BAX, membrane isoform alpha; BAXA; Bcl2-associated X protein

• Specificity Sensitivity:

  Bax (Phospho-Ser184) antibody detects endogenous levels of Bax only when phosphorylated at serine 184.

• References:

  Oltvai Z.N., Cell 74:609-619(1993). Apte S.S., Genomics 26:592-594(1995). Shi B., Biochem. Biophys. Res. Commun. 254:779-785(1999).

• All Sites:

  Human: Ser184; Mouse: Ser184; Rat: Ser184

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using Bax (Phospho-Ser184) antibody. Western blot analysis of extracts from K562 cells, using Bax (Phospho-Ser184) antibody.

Bax (Phospho-Ser184) antibody reacts with epitope- specific phosphopeptide and corresponding non- phosphopeptide. The absorbance readings at 450 nM are shown in the ELISA figure.