• Catalog Number:

  E1A0016A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Keratin 18 around the phosphorylation site of serine 33 (A-A-SP-V-Y).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human

• SwissProtNo:

  P05783

• Applications:

  WB: 1:500~1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:5000

• Alter Name:

  CK 18, CK18, CYK18, Cytokeratin endo B, K18, K1C18, KERD, KRT1-18, KRT18, Keratin, Keratin D, Keratin, type I cytoskeletal 18, cytokeratin 18, type I cytoskeletal 18

• Specificity Sensitivity:

  Keratin 18 (phospho-Ser33) antibody detects endogenous levels of Keratin 18 only when phosphorylated at serine 33.

• References:

  J Liao, J. Cell Biol., Dec 1995; 131: 1291. CF Chou and MB Omary, J. Biol. Chem., Feb 1993; 268: 4465 - 4472. NO Ku and MB Omary, J. Cell Biol., Oct 1994; 127: 161. Nam-On Ku J. Cell Biol., May 2000; 149: 547.

• All Sites:

  Human: Ser33

P-peptide - +

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Keratin 18 (phospho-Ser33) antibody. Western blot analysis of extracts from HT29 cells, using Keratin 18 (phospho-Ser33) antibody.

P-peptide - + Immunofluorescence analysis of HeLa cells, using Keratin 18 (phospho-Ser33) antibody (#11316).