Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human ATRIP around the phosphorylation site of serine 224 (H-V-SP-P-R).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Reactivity:
Human (Identities = 100%, Positives = 100%)
SwissProtNo:
Q8WXE1
Applications:
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000
Other Names:
ATIP; ATM and Rad3 related interacting protein; ATR-interacting protein
Specificity Sensitivity:
ATRIP (Phospho-Ser224) antibody detects endogenous levels of ATRIP only when phosphorylated at serine 224.
References:
Cortez D., Science 294:1713-1716(2001).
Ota T., Nat. Genet. 36:40-45(2004).
Mural R.J., Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
All Sites:
Human: Ser224
P-peptide - +
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using ATRIP (Phospho-Ser224) antibody.
Western blot analysis of extracts from 292 cells, treated with UV (15mins), using ATRIP (Phospho-Ser224) antibody.