• Catalog Number:

  E012050-1

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Tsc2 around the phosphorylation site of threonine 1462(G-Y-TP-I-S).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P49815

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:4000

• Specificity Sensitivity:

  Tsc2 (phospho-Thr1462) antibody detects endogenous levels of Tsc2 only when phosphorylated at threonine 1462.

• References:

  Cai SL, et al. (2006) J Cell Biol 173, 279-89. Roux PP, et al. (2004) Proc Natl Acad Sci USA 101, 13489-94. Li Y, Inoki K, Vacratsis P, Guan KL (2003) J Biol Chem 278, 13663-71. Manning BD, et al. (2002) Mol Cell 10, 151-62. Goncharova, E. et al. (2002) J. Biol. Chem. 277, 30958-30967. Aicher, L. D. et al. (2001) J. Biol. Chem. 276, 21017-21021.

P-Peptide - + Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using Tsc2 (phospho-Thr1462) antibody (E012050).